Yuri (ユリ) is a main character of Doki Doki Literature Club!. She is the third member of the Literature Club, and a character whom the player can write poems for.

Yuri is a moderately tall, statuesque 18-year-old[1] adolescent young woman. She is the tallest female member of the Literature Club—with a fair-toned complexion, and a comparably developed physique in contrast to that of the other girls, possessing a slender bodily shape and a fairly sizable bosom. According to the Protagonist, Yuri's movements are surprisingly elegant due to her long legs. It is also implied that Yuri has a bit of a posture problem, as stated in-game by her and the Protagonist during their conversation in Act 2, causing her to have some back pain issues.

Yuri has reflective, pin-straight dark purple hair that flares out at the bottom and ends at her thighs. Followed with a pair of two prominent forelocks that lengthen down to her chest-height, framing her face on either side. A Violet barrette is also attached at the upper right of her hair strand. Her eyes are normally lidded and intense, having sparkly irises colored the same as her hair, but a smidgen lighter.

While in school, Yuri wears the standard issue school uniform: consisting of a white collared top which wears thin red string at the collar and is shaped into a bow tie, followed by a mocha-brown knit vest, a fully tucked and buttoned warm gray blazer, a pleated dark blue skirt, calf-high stockings, and white uwabaki slippers with sky blue fronts.

If the player decides to help out with Yuri over the weekend in Act 1 to make posters for the upcoming Festival, Yuri wears a gray beige[4] vertical knit turtleneck sweater alongside a pair of fitted black leggings. Footwear is not shown.

Monika (モニカ) is a main character and the poster girl of Doki Doki Literature Club!. She is the President and Founding Member of the Literature Club. As the game's driving force, she directs club members to write poems until the club festival, which enables and develops the characters and story.

Monika is a fair-skinned 18-year-old[1] adolescent young woman—as described through the eyes of the Protagonist through her introduction, as "beautiful" and "athletic", suggesting she's generally physically fit and well-proportioned compared to the other girls. Her physique is portrayed to be of average stature, being the second/third tallest member of the Literature club right behind Yuri or the Protagonist, with a slender build and an ample bust.

Monika has extremely long, glistening coral-brown[3] hair that is perpetually tied up into a high-hoisted ponytail which extends way past her navel that curls up at the ends, kept fastened by a large white ribbon. Her fringe ends at eyebrow height and her face is framed between two chest-length forelocks. Her eyes are calm and sparkly, colored emerald-green.

In terms of attire, Monika wears the standard-issue school uniform consisting of a collared white top layered under a knitted mocha-brown sweater vest and fully buttoned periwinkle-gray blazer. Followed along with a pleated blue skirt, long sleek pantyhose thigh-high stockings, and white uwabaki slippers with pastel-pink fronts. At the center of her collar, she has a thin red string wrapped around it, shaped like a bow tie.

Natsuki (ナツキ) is a main character of Doki Doki Literature Club!. She is the fourth member of the Literature Club, and a character whom the player can write poems for.

Natsuki is a notably short-statured, 18-year-old[1] young woman, frequently referred throughout gameplay by other characters as having an outwardly "cute" appearance; having perpetually flushed cheeks and a fair-toned skin color. Her canine teeth are longer than the rest of her teeth, making them visible when she opens her mouth wide enough. She is the shortest member of the Literature Club, a particular feature that results in people commonly mistaking her for a first year student. In Act 2, Monika suggests that Natsuki's height is due to a case of malnutrition—because of the apparent abuse from her father, Natsuki's growth has become significantly stunted as a result. Following this, Natsuki is portrayed to have a bit of an underdeveloped physique for her age; having a thin, broad-shouldered build and a flat chest.

Natsuki has fringed, bobbed Pastel-Pink hair lengthening to her chin. Her bangs are predominantly swept to the right, having two prominent strands of hair framing either side of her cheeks. Her hair accessories consist of a red anime~esque X hairclip, paired along by two red ribbons holding up two small matching pigtails on either side of her head, with the rest of her hair left down. Her eyes are glittery and colored the same as her hair, and her face is often worn in a sour or pout-y expression.

While in school, Natsuki wears the standard-issue female school uniform: consisting of a warm gray blazer fully buttoned and untucked, a trimmed mocha-brown sweater vest, a collared white shirt slightly unbuttoned at the collar with a thin red ribbon attached at the collar, a dark blue pleated skirt, thin white calf-high socks and white uwabaki slippers with sky-blue fronts.

If the player decides to help out with Natsuki over the weekend in Act 1 to bake cupcakes for the upcoming Festival, Natsuki wears a puff-sleeved white shirt with a small light pink cat face emblem on the left side, paired with light pink trims lining the edges of the sleeves. Tucked under by a tri-tiered frilled light pink tutu with a thin black trim at the top hemline. Under the shirt, two black bra straps are visible on her shoulders. Footwear is not shown.

Sayori (サヨリ) is a main character of Doki Doki Literature Club!. She is the Vice President and second member of the Literature Club, the protagonist's childhood friend and a character whom the player can write poems for.

Sayori is a petite and rosy-cheeked 18-year-old adolescent young woman of an outwardly bubbly and subtly unkempt appearance; officially standing as the second shortest member of the Literature Club, right ahead of Natsuki by a mere three , portrayed as having a slim physique and a small bust.

Sayori has shiny, coral-pink[3] hair fashioned into a chin-length bob, slightly messy due to her disuse of brushing it, having her fringe parted into three separate parts along her forehead; with a noticeable tuft right between her eyebrows, while the rest curls inwards around her cheeks, framing her face. Sayori's trademark large red bow adorns the top left corner of her hair. Her eyes are noticeably wide and well-rounded, with sparkly sky-blue irises.

While at school, Sayori wears the standard-issue school uniform: a white collared top with a thin red ribbon-like string tied around the collar. Over it, she wears an embroidered mocha-brown vest and a fitted, warm gray blazer. She leaves the blazer fully unbuttoned, revealing a small portion of the vest. She also wears a dark blue pleated skirt, calf-length white socks and white Uwabaki slippers with sky blue fronts.

At home, Sayori wears a pink T-shirt with the sleeves rolled up to her elbows and bluish-gray shorts with small ruffles at the bottom. Footwear is not seen.

At the scene of her , Sayori is found barefoot, hanging from a noose. Her skin pallor, her eyes dull and her fingertips visibly bloodied. She was wearing a two-tone off-white and light brown collared dress shirt, the left side of which had slipped off her shoulder, exposing her collarbone. Her dark red shorts had a thin white line running vertically downwards and two matching drawstrings.