The Daughters of Aku were a group of seven Human-demon hybrid women who were born and raised by their mother, The High Priestess, for the sole purpose of killing Samurai Jack. They are from a cult of the same name dedicated to Aku and appear during the events of Season 5. As of Episode XCV, only Ashi was confirmed to be alive. It was confirmed in Episode XCVIII that all the Daughters, minus Ashi, did indeed perish by Jack according to the High Priestess. In Episode C, it was revealed that they were indeed the daughters of Aku with their mother having taken the essence of Aku and birthing them as a result.

After Jack and Ashi time-traveled into The Past shortly after Aku originally hurled Jack into The Future, Jack finally triumphed in destroying Aku. With his demise in the past, Ashi (And the rest of her sisters) are rendered non-existent and thus were never born. In Samurai Jack: Battle Through Time, the time pockets the demon himself created of the future not only affected by space or time, but prevents their non-existence allowing Ashi to be the only survivor in the game's alternate ending as Jack's wife.

Appearance

Being septuplets, they all shared very similar physical traits consisting of black hair, narrow eyes and what appeared to be pitch-black latex-like suits. It is later revealed that their suits are in fact solidified tar as a result of being pushed into a hot, tar-like substance from the Pit of Hate by their mother. The only distinguishing trait among them is their varying hairstyles. When out on their mission, they wear white masks with black horns made to resemble Aku's.