Characters: Hellboy, Professor Trevor "Broom" Bruttenholm, Alice Monaghan, Major Ben Daimio, Vivienne Nimue / The Blood Queen, Gruagach, Baba Yaga, Ganeida, Lady Hatton, Lord Adam Glaren, King Arthur, Merlin, Lobster Johnson, Grigori Rasputin, Karl Ruprect Kroenen, Agent Esteban Ruiz / Camazotz, Agent Madison, Agent Strode, Agent Taylor, the B.P.R.D. , Margaret Monaghan (Alice's Mother), Mr. Monaghan (Alice's Father).

Locations: Tijuana (Mexico), the B.P.R.D. Headquarters (Colorado), London (England), the "Codswallop" fish and chips shop secret entrance, O'Toole’s Tavern, the Osiris Club Estate, Pendle Hill (Lancashire), the Isle of Vance (Scotland), Baba Yaga’s Dimension (Siberia), the underground cave system housing the Blood Queen’s Tomb, and Wells Cathedral.